Dr Amber Afroz
Designation : Assistant Professor
Specialization : Biochemistry
Specialization : Biochemistry
Email : [email protected]
Office Number : +(92) 3359483609
I am Dr. Amber, originally from Pakistan, currently serving as an Assistant Professor in the Department of Biochemistry and Biotechnology (BAB) at the University of Gujrat (UOG). With over thirteen years of experience in academia under the TTS, and BPS system, I have made significant contributions to research and education. I successfully led the NRPU Project 6506, a high-impact research initiative funded by HEC with a budget of 13.3 million PKR —the highest awarded by NRPU in 2017. This project focused on proteomics analysis to identify RNAi targets in the Gujrat District. During the study, I optimized 2D-PAGE for aphid and wheat proteins, identifying differential protein-encoding genes as RNAi targets. The findings resulted in five research publications and contributed to cloning efforts for aphid-resistant wheat. Throughout my career, I have supervised five PhD students and co-supervised two, in addition to mentoring over 40 MPhil students. My research contributions include approximately 40 publications in reputed journals. During my PhD, I conducted differential proteomics analysis of tomato cultivars for biotic stress resistance. Additionally, I worked on tissue culture optimization for in-vitro tomato growth and the development of bacterial wilt-resistant tomato cultivars. My expertise spans biotechnology, proteomics, genetic engineering, and plant stress resistance, making a significant impact on both research and academic mentorship.
Biotechnology (RNAi, Agrobacterium-mediated transformation in dicots & monocots), PCR, RT PCR, Southern Blot, Western Blot analysis. In-vitro techniques (Plant tissue culture, Micropropagation), Proteomics (Protein extraction, 2D PAGE, Mass spectrometry), Expression analysis.
- PhD,Quaid-e-Azam University Islamabad
- MPhil,Quaid-e-Azam University Islamabad
- MSc,University of Agricultural Faisalabad
- PhD Scholarship HEC IRSIP Scholarship
- One Year at UOG as Assistant Professor IPFP
| Student Name | Degree | Title | Status / Completed Year |
|---|---|---|---|
| Ghulam Abbas | BS | Expression analysis of ATP binding cassette transporters, ATP synthase, and chaperonins in response to salicylic acid treatment in tomato - | 2013 |
| Asma Rafique | Ph.D | RNA interference-mediated silencing of laccase gene in Triticum aestivum cultivars against indigenous Sitobion avenae The presence of Sitobion avenae, a major insect pest, poses a significant threat to the productivity of Triticum aestivum L., resulting in substantial yield loss. This study aimed to evaluate the potential of laccase (lac) as a target for RNA interference (RNAi) against S. avenae. A preference test was conducted to select aphid susceptible wheat cultivars (Anaj 2017, Galaxy 2012, and Punjab). RT-PCR confirmed the presence of S. avenae laccase (Salac) and the amplified sequence was assigned the accession number “ON703252” by GenBank. To downregulate Salac expression, an RNAi approach was employed using theERNAi tool, which facilitated the design of 143 siRNA and one dsRNA target. In a dsRNA feeding assay, S. avenae exhibited a significantly higher mortality rate of 69% compared to the control group. Moreover, an 8-day feeding of lac dsRNA resulted in a significant 61% reduction in lac expression. A dsRNA targeting Salac applied as a spray, facilitated systemic translocation from leaf to the root, with the highest lac expression detected in the root, followed by stem and leaf within a 9-13 day period post spray. For lac silencing, RNAi-GG, employing a single restriction ligation reaction was utilized through a golden gate cloning strategy, enabling efficient lac gene silencing. Successful transformation of wheat was achieved using in planta and tissue culture methods with various explant sources, including seedlings, apical meristems, and calli. In the Agroinfiltration experiment, the highest transformation efficiency was observed in Galaxy 2012 (16%), followed by Anaj 2017 (13%) and Punjab (8%). Additionally, aphid bioassay conducted on transgenic and non-transgenic wheat cultivars revealed significant differences in aphid reproduction and survival rates between the two groups. Optimization of tissue culture techniques showed that a combination of 3mg/l of 2, 4-D, and 10% coconut water resulted in maximum callus formation, while 1.5 mg/l of BAP and 10 % coconut water led to the highest regeneration. In tissue culturetransformation, Galaxy 2012 exhibited the highest transformation efficiency (1.5%) followed by Anaj 2017 (0.8%) and Punjab (0.2%). Notably, in planta transformation yielded better results, with a maximum transformation efficiency of 16% in Galaxy 2012 and a minimum of 5% for Punjab. Furthermore, a combination of 250µM acetosyringone and 3 hours of co-cultivation resulted in the highest transformation efficiency. Gene expression analysis using qRT-PCR with actin as the internal control revealed relative Salac expression levels of 0.7 in Galaxy 2012, 0.5 in Punjab, and 0.3 in Anaj 20p17, showing a significant decrease. In the aphid bioassay, transgenic Galaxy 2012 exhibited the highest S. avenae mortality rate (4/14 aphid) compared to positive control aphid-resistant Zincol 2016. The findings of this study demonstrate the efficacy of RNAi-mediated targeting lac as an effective approach for managing S. avenae. These results have significant implications for the development of insect pest management strategies in wheat farming. | 2024 |
| Atif Mehmood | Ph.D | Production of insect and herbicide-resistant transgenic maize by fusion transformation Maize an important cereal crop is affected by herbicide and insects. The research work is focused to develop the resistance against Ostrinia furnacalis, Helicover paarmigera and Mythima separate and herbicide is induced in maize through fusion transformation. Callus induction and regeneration of maize cultivars: Neelam, Dehkan, and Pak Afghoi mature embryos, immature embryos, and leaf discs were optimized as a prerequisite. The highest callus induction was achieved with 2,4-D (3mgL -1 ), and Benzyl Amino Purine (BAP) (2mgL - 1 ) and 20% coconut water by mature embryos: Dehkan (80%), followed by Neelam (68%), and Pak Afghoi (62%). Callus induction by leaf discs was highest by Neelam (60%), Dehkan(56%) and Pak Afghoi (42%) after 25D. 2, 4-D (0.5mgL -1 ), in combination with BAP (2 mgL -1 ) presents maximum regeneration efficiency in combination to 20% coconut water: 60, 54, and 43% for Dehkan, Neelam, and Pak Afghoi respectively. Fusion transformation was done by binary vector pCAMBIA1301. Glyphosate resistant gene EPSPS was placed at site of hygromycin under the enhanced CAM35S promoter and CAMV poly (A) signal, and pest resistant Cry1Ac was cloned at GUS site under the CAM35S promoter, and NOS terminator. Recombinant pCAMBIA 1301 was transformed in LBA4404 for In planta, and tissue culture transformation. Tissue culture transformation efficiency was 0.9% for Pak Afghoi, followed by Dehkan 0.6%, and Neelam 0.5%. In planta transformation was proved to be better thantissue culture: and induce 6.2% transformation in Pak Afghoi, followed by Dehkan (5.2%), and Neelam (4.2%). Acetosyringone (50uM-250um) have significant impact on transformation efficiency. 50uM acetosyringone gave the lowest transformation efficiency While maximum transformation efficiency was obtained by 250µM acetosyringone: Pak Afghoi (10%), Neelam (8%), and Dehkan (9%). Co-cultivation period was tested from 2-8h in tissue culture transformation and best response was with 2 hours of incubation. Transformation efficiency observed was 8.5, 7.2, and 6.4% for Neelam, Dehkan Pak Afghoirespectively. Co-cultivation period augmentation resulted in decreased transformation efficiency: 0.7% in Neelam, 0.6% by Dehkan and 0.8% by Pak Afghoi. Glyphosate assay at the dosage of (0.05, 0.1, 0.15, & 0.2%), was used and transgenic plants survived up to 0.1% glyphosate. A quantitative insect assay was performed with 4mg invaded insects for all cvs: and Pak Afghoi gave best response with survival of 1.8 mg insects, followed by Dehkan (2mg), and Neelam (2.7mg) 6D-post infestation. Quantitative Reverse Transcriptase PCR (qRT-PCR) displayed maximum EPSPS and Cry1Ac expression by Pak Afghoi (0.8%),followed by Neelam (0.5%) and Dehkan (0.3%) and Cry1Ac expression was maximum in Pak Afghoi 0.7%, followed by Neelam and Dehkan in comparison to no expression in control. Southern blot analysis showed two copies of T-DNA in Pak Afghoi: while single copy number insertion for Dehkan and Neelam. The chi-square test displayed the Mendelian genetic ratio of 3:1 for insect and glyphosate resistance. | 2024 |
| Javeria Shafqat | Ph.D | Differential Proteomics of Wheat Aphids for Identification of RNA Interference Targets Differential proteomics of wheat aphids for identification of RNA interference targets | 2024 |
| Zamarrah Manzoor | MS | Investigation of vitamin D binding protein, and retinol levels in Alzheimers disease - | 2024 |
| AAYEFA EHSAN | MS | AAYEFA EHSAN Antibiosis and antixienosis assays were performed to categorize resistant and susceptible wheat varieties. Anaj was most susceptible, while Fakhar E Bakhar was found highly resistant among five wheat varieties tested. RNA interference (RNAi) works well at controlling pest populations and safeguarding crops. Odorant binding protein 3 (OBP3), and chitin synthase (CHS) in wheat aphids are used as RNAi targets. CHS catalyses synthesis of chitin, which is essential structural component of aphids, present in exoskeleton and digestive tract. OBPs are essential for detecting odours during mating, feeding, host hunting, and oviposition. RNA was extracted from Sitobion avenae and Rhopalosiphum padi ufollowed by cDNA synthesis for PCR amplification of OBP3 and CHS. qPCR was performed to check the expression level of genes. For dsRNA feeding assay, cDNA was used for gene amplification of genes. Primers for T7 promoters were used for amplification of CHS and OBP3 gene amplification. DsRNA-CHS and DsRNA-OBP3 were delivered as diet to the aphids to observe their affect. Mortality rate of aphids was observed for 8 days. DsRNA-CHS showed 75% mortality in S. avenae and 60% mortality in R. padi, while dsRNA-OBP3 showed 65 and 55% mortality in S. avenae, and R. padi respectively. It is concluded that CHS, and OBP3 can be effective RNAi targets and can be used as a bio pesticide to control aphid population. | 2024 |
| Mariyam Riaz | MS | DIFFERENTIAL EXPRESSION ANALYSIS OF ACETYLCHOLINESTERASE AND CATALASE GENE AS RNA INTERTETFERENCE TARGETS AGAINST Sitobion avenae Triticum aestivum is prone to attack by various pathogens such as aphids. In this study five wheat cultivars named as Anaj, Fakhar-e-Bakhar, Subhani, Akhbar, and Dilkash were used. Antixenosis and Antibiosis experiments show that Anaj is the most resistant variety and least susceptible. The nature of wheat cultivars was recognized and based on information the local wheat cultivars were identified as either susceptible or resistant to S. avenae infestation. Catalase gene (CAT), and Acetylcholinesterase gene (SaAce1) will be the potential targets in different types of aphids for RNAi technology with feeding assay and microinjection. RT-PCR confirmed the presence of these genes post-feeding with gene-specific primers. RNA is extracted by using S. avenae. Specific primers designed for Acetylcholinesterase and catalase genes were used in PCR for amplification of required genes. RT-PCR shows CAT (837 bp), and SaAce1 gene (801 bp). qPCR shows the enhanced expression of CAT, and SaAce1 post-feeding, while no expression was observed pre-feeding.. dsRNA feeding assay done for the catalase and acetylcholinesterase genes. The highest survival rate of S. avenae was found on 20% sucrose; so it was taken as standard in this diet. Aphids were fed on dsRNA catalase and dsRNA acetylcholinesterase genes. Highest expression as compared to other wheat cultivars. Ct value for catalase is 23 and for acetylcholinesterase is 22. Expression for acetylcholinesterase is high. | 2024 |
| HADIA IMTIAZ | MS | UTILIZATION OF CARDIOLIPIN AND BIOTIN SYNTHASE AS RNAI TARGETS AGAINST Sitobion avenae RNA interference has emerged as a major strategy for the aphid challenge however, limited practices in modifications of genome and protection of genetically modified organisms are considered as a major concern. Sitobion avenae F.is one of the major agricultural pests, destroying wheat crops and inducing plant viruses in them. The objective of this study was to find potential RNAi targets against the said aphid to silence the specified genes. Genes for cardiolipin synthase (cls) of 828 bp expressed in mitochondria and biotin synthase (bioS) of 601 bp expressed for metabolic pathways are recognized as potential targets in the endosymbiont bacterium Buchnera aphidicola of the said aphid. RNA was extracted from S. avenae followed by complementary DNA (cDNA) synthesis which was followed by Polymerase Chain Reaction (PCR). Bioinformatics tools were used to verify promising RNAi targets in the said genes. This was followed by double-stranded RNA (dsRNA) synthesis. To check the silencing effect and mortality rate in the said aphids, a dsRNA assay was performed via feeding them on artificial diet of sucrose. The targets were confirmed via qRT-PCR which showed lower expression in treated samples in comparison to the control samples and caused 88% and 85% reduction of bioS and cls genes’ expression respectively. These findings demonstrated the potential of said genes to reduce the aphid population and improve crop protection, providing a foundation for RNAi-based pest control strategy in wheat. Future research should aim to refine delivery methods and assess long-term field effects | 2024 |
| Shiv Ram Ashraf | Ph.D | Extraction and characterization of peroxidase from indigenous plants and fungi Aspergillus niger is reported to be favorable for commercially useful enzymes including peroxidase (POX) production. In this study, POX was extracted from 20 different plants, callus, along with Aspergillus strains, and their activity was compared. From the 20 plants tested; the best activity was found in Brassica oleracea (2.65 U/ml). Amongst the callus Solanum lycopersicum cv, Riogrande showed maximum activity (2.8 U/ml); while A. niger strains displayed maximum activity (7.17 U/ml). High POX-producing A. niger strains were identified by 18S RNA phylogeny, and the sequences obtained were submitted to GenBank:IDs allotted. Eight different substrates were screened out for POX activity and corncob was found most favorable, with the highest POX activity. A. niger strains were compared to other Aspergillus strains by phylogenetic analysis. Phylogenetic analysis of respective strain presented their alignment with A. niger, A. fumigatus, A. awamori, and A. luchuensis. POX isolated from the BMB17 exhibited maximum activity (7.17U/ml). The POX sequence (Cytochrome C (Cyt) (1734 bps)+ Manganese (Mn) POX (1314 bps) was amplified and sequenced for its confirmation. 12.5% SDS (sodium dodecyl sulphate) PAGE (polyacrylamide gel electrophoresis) revealed a 40kDa POX band with standard molecular weight markers. The POX was immobilized using different concentrations of sodium alginate and maximum activity was observed with 3% sodium alginate. The BMB-17 and BMB-18 with maximum POX activity was enhanced by solid-state fermentation (SSF). The highest POX activity was obtained with pH 6.5, temperature 37 o C, and incubation period of 5 D. Characterization for enhanced POX production was done by optimizing pH, substrate amount, temperature, incubation days, modulator, inhibitor, activators and solvents. From metal ions Ca +2 increased the POX activity, while the Zn +2 , Cu +2 and Mn +2 had the inhibitory effect. Modulators EDTA, urea and tween (polysorbate) also had inhibitory effect on the POX activity along with solvents (acetonitrile, ethanol and propanol). BMB-17 POX was purified up to 7.29 folds by ammonium sulfate, and size exclusion gel chromatography with a specific activity of 12.15 U/mg. Km and Vmax of purified POX were 1.57mg/ml, and 7.75 umol/ml/min respectively. POX application was checked for textile dye decolorization, tomato and orange shelf-life improvement, waste water management and bread fluffing. The combined effect of identified Aspergillus strains MN611114.1 (BMB 17), and MN559756.1 (BMB-18) enhanced the POX activity (Cyt+ Mn POX) in comparison to individual activities in textile dyes decolorization (98.3%). In industrial water waste management, the value ofbiochemical oxygen demand is decreased to 35%. Fourier transform infrared spectroscopy analysis displayed peaks of 1249 cm -1 , and 1025 cm -1 (Aromatic Ether, aryl-O stretch) disappeared after POX treatment. After 28D POX (50 U) treated tomato and orange improved shelf life was observed in comparison to control. It was followed by POX effectiveness in the bread trait. It gave bread best quality with its fluffing. | 2023 |
| Reema Aftab | Ph.D | Therapeutic potential of Mentha piperita Copper and Silver Nanoparticles for Chronic Wound Healing Therapeutic Potential of Mentha piperita Copper and Silver Nanoparticles for Chronic Wound Healing | 2023 |
| Eijab Afzal | MS | Comparative Proteome Analysis of Triticum aestivum local Cultivars in Response to Aphid Infestation - | 2023 |
| Rabia Noreen | MS | Differential analysis of lipoxygenase and pathogenesis-related protein-4 of Triticum aestivum post Sitobion avenae feeding - | 2023 |
| Namra Mubashir | MS | Antidiabetic potential of Moringa oleifera and Asteracantha longifolia - | 2023 |
| Ruhma Mukhtar | MS | Differential proteome analysis of Sitobion avenae post-Triticum aestivum cvs. leaf feeding - | 2023 |
| Foqia Tasneem | MS | Determination of pharmacological significance of bioactive Compounds from avocado and bitter melon - | 2023 |
| Fatima Akbar | MS | Biosynthesis and Characterization of Silver Nanoparticles from Mentha piperita Extract and their Inhibitory Analysis - | 2022 |
| Sheraz Khalid | MS | Expression analysis and mutation detection after RNA Interference of Rhopalosiphum padi voltage-gated sodium channel gene - | 2022 |
| Raseefa Afzal | MS | Production and Characterization of Pectin methyl Esterase from Aspergillus niger - | 2022 |
| Muqaddas Ali | MS | RNA Interference mediated Knockdown of the Voltage-Gated Sodium Channel (RPNAV); gene causes mortality in Rhopalosiphum padi - | 2022 |
| Freeha Shahbaz | MS | Identification and In Silico Analysis of Missense SNPs of Human Genome Related to Psoriasis Disease in South Asian Population - | 2022 |
| Qudoosia Mehmood | MS | Codon optimization of CRY Gene Family for enhanced insect tolerance in Maize - | 2020 |
| Noor ul Ain | MS | Comparative protein analysis of Sitobion avenae and Ropalosiphum padi before and after infesting wheat cultivars - | 2020 |
| Ayesha Hameed | MS | Expression analysis of wheat cultivars proteins susceptible and resistant to cereal aphids - | 2020 |
| Mariam Fatima | MS | Determination of Defense Genes Expression in Response to Insect Wounding and Jasmonic Acid Application in Tomato - | 2019 |
| Iqra Mahmood | MS | Confirmation of Mp58 and OBP2 Genes and their Role as RNA Interference Targets in Local Peach Potato Aphids - | 2019 |
| Safeena | MS | Evaluation of Odorant Binding and Heat Shock Protein Encoding Unigenes As Promising RNA Interference Targets in Myzus persicae - | 2019 |
| Sara Mehmood | MS | Determination of Diabetic Markers Expression after Treatment with Aloe Vera and Azadirachta Indica - | 2019 |
| Farasat Ayesha | MS | Investigation of Stress-Related Genes Expression in response to Jasmonic Acid Application and insect Herbivore attack - | 2019 |
| Hafeez ur Rehman | MS | Investigation of Suitable Biomarkers for the Early Diagnosis of Parkinsons Disease - | 2019 |
| Shehr Bano | MS | Characterization of a salivary transcript from Indigenous potato aphid, Myzus persicae - | 2018 |
| Khushbu Zara | MS | Comparison of Aerial Parts and Fruits of Azadirachta Indica for their Anti-Diabetic Potential - | 2018 |
| Kinza | MS | Assessment of Aloe Vera Gel for Its Oxidative Stress and Anti-Diabetic Potential - | 2018 |
| Hadiqa Tehreem | MS | To identify RNAi Targets in Grain Aphids for the Protection of Wheat in Hot Tropical Regions of Punjab - | 2018 |
| Neelum Akram | MS | Identification of Possible RNAi Targeted Gene and Its Computational and Molecular Analysis in Grain Aphids (Sitobion Avenae - | 2018 |
| Zoma Chaudhry | MS | Characterization and Computational Analysis of Novel Grain Aphid Unigenes Residing on Triticum Aestivum cvs - | 2018 |
| Arooshia Shahid | MS | Identification of Potential RNAi Targets in Wheat Grain Aphid Species by Using Molecular and Computational Analysis in Gujrat, Pakistan - | 2018 |
| Ghulam Abbas | MS | Detection of P53 codon72 and 248 polymorphism and association with prostate cancer risk in Pakistani population - | 2016 |
| Maria Hashmi | MS | Identification of gut-specific sequences in the local population of aphids -- | 2016 |
| Muhammad Nauman | M.Sc. | Augmentation of drought tolerance in Triticum aestivum cvs by ATP synthase gene isolated from Solanum lycopersicum - | 2016 |
| Atif Mehmood | MS | Production of glyphosate tolerant transgenic maize lines - | 2015 |
| Waqar Younus | MS | Detection of virulence genes and antibiotic resistance testing of salmonella serovars isolated from urban drinking water in District Gujrat, Pakistan - | 2015 |
| Asma Rafique | MS | Transgenic Wheat Expressing A Codon Optimized Synthetic EPSPS Confers Glyphosate Tolerance - | 2015 |
| Javeria Shafqat | MS | Hepatoprotective effect of penta-herbal ethanolic extracts against CCl4 induced hepatic damage in albino rats - | 2015 |
| Mobeen Akhtar | MS | Identifying Paenibacillus polymyxa AM-44 from unpasteurized milk using 16S rRNA and detecting class IIa YGNGV Pediocin PA-1 (AcH) like bacteriocin by PCR - . | 2014 |
| Anjala Ayoub | MS | Stress protein expression in response to Jasmonic acid, Cabbage looper (Trichoplusia ni) & Wounding - | 2014 |
| Saba Saleem | MS | Identification of Protein biomarkers for early detection of hepatocellular carcinoma - | 2014 |
| Amna Tanveer | M.Sc. | Determination of Genes Induced In Response to Salicylic Acid in Solanum lycopersicum cv Riogrande - | 2012 |
-
1. Ali MA, Khan MAU, Naz H, Malik MAM, Rashid U, Ashraf NM, Afroz A, Shafiq M, Rao AQ “CAS9 mediated In-Planta defence Strategy against tomato leaf curl new Delhi virus (ToLCNDV) in tomato” Journal Of Phytopathology, January 2025 DOI: https://doi.org/10.1111/jph.70026
-
2. Mukhtar R, Afzal E, Noureen R, Nadia Zeeshan N, Afroz A “Comprehensive Insights into Sitobion avenae preferences and performance on Pakistan’s wheat cultivars leading to identification of potential RNAi targets” Sains Malaysiana, September 2024 DOI: http://doi.org/10.17576/jsm-2024-5310-09
-
3. Shafqat J, and Afroz A* “RNA Interference of Sitobion avenae Voltage-gated Sodium Channels for improved grain aphid resistance” International Journal Of Tropical Insect Science, April 2024 DOI: https://doi.org/10.1007/s42690-024-01261-1
-
4. Akhtar MA, Butt MQS, Afroz A, Rasul F, Irfan M, Sajjad M, Zeeshan N “Approach towards sustainable leather: Characterization and effective industrial application of proteases from Bacillus sps. for ecofriendly dehairing of leather hide” International Journal Of Biological Macromolecules, March 2024 DOI: https://doi.org/10.1016/j.ijbiomac.2024.131154
-
5. Aftab R, Akbar F, Afroz A*, Asif A, Khan MR, Rehman N, Zeeshan N “Mentha piperita silver nanoparticle loaded hydrocolloid film for wound restoration in diabetic rats and identification of bioactive compounds by ESI MS/MS analysis” Journal Of Wound Care, March 2024 DOI: https://doi.org/10.12968/jowc.2024.33.Sup3a.xlviii
-
6. Shafqat J, Afroz A* “Differential protein expression analysis of wheat cultivars and grain aphids post-feeding” Journal of Tianjin University Science and Technology, January 2024 DOI: https://doi.org/10.5281/zenodo.10612560
-
7. Adeeba Tabassum, Amber Afroz, Nadia Zeeshan “Unveiling the Therapeutic Potential of Annelids as Thrombolytic Agents: A Research Investigation” Journal of Xian Shiyou University, Natural Science Edition, January 2024 DOI: http://xisdxjxsu.asia
-
8. Farooq M, Ahmad R, Shahzad M, Rehman SU, Sajjad Y, Hassan A, Shah MM, Afroz A, Khan SA “Real-time expression and in silico characterization of pea genes involved in salt and water-deficit stress” Molecular Biology Reports, December 2023 DOI: https://doi.org/10.1007/s11033-023-09064-2
-
9. Irfan M, Bagherpour S, Munir H, Perez-Garcia L, Abelha TF, Afroz A, Zeeshan N, Rashid U “C–MS metabolomics profile of methanol extract of Acacia modesta gum and gum-assisted fabrication and characterization of gold nanoparticles through green synthesis approach” International Journal Of Biological Macromolecules, August 2023 DOI: https://doi.org/10.1016/j.ijbiomac.2023.126215
-
10. Ashraf SR, Afroz A*, Anwar Z “ Physicochemical parameters optimization and peroxidase characterization from Aspergillus niger native strain by solid-state fermentation for improved dye decolorization” Bioresources, June 2023 DOI: https://doi.org/10.15376/biores.18.3.5512-5530
-
11. Aftab R, Afroz A*, Zeeshan N “Diabetic wound healing with enhanced antimicrobial activity against methicillin-resistant Staphylococcus aureus by native Mentha piperita copper nanoparticles” Journal of Tianjin University Science and Technology, June 2023 DOI: https://doi.org/10.17605/OSF.IO/A8HW9
-
12. Rafique A, Afroz A*, Zeeshan N, Rashid U, Khan MAU, Irfan M, et al. “Production of Sitobion avenae-resistant Triticum aestivum cvs using laccase as RNAi target and its systemic movement in wheat post dsRNA spray” Plos One, May 2023 DOI: https://doi.org/10.1371/journal. pone.0284888
-
13. Akhtar MA, Afroz A, Zeeshan N “Purification Characterization and Optimization Fermentation Parameters of alkaline protease enzyme produced from Bacillus subtilis strain BS.HK” Journal of Xian Shiyou University, Natural Science Edition, January 2023 DOI: http://xisdxjxsu.asia
-
14. Mehmood A, Afroz A*, Rashid U “An efficient regeneration with multiple shoots from mature embryo-derived callus of Zea mays cvs” Journal of Xian Shiyou University, Natural Science Edition, June 2022 DOI: http://xisdxjxsu.asia
-
15. Rafique A, Afroz A*, Zeeshan N “Enhanced in-vitro embryogenesis and multiple shoot regeneration of wheat local cvs (Triticum aestivum L.) with coconut water” Journal of Xian Shiyou University, Natural Science Edition, June 2022 DOI: http://xisdxjxsu.asia
-
16. Rasool F.; Uzair M, Naeem MK, Rehman N; Afroz A, Shah H, Khan MR “Phenylalanine Ammonia-Lyase (PAL) Genes family in wheat (Triticum aestivum L.): Genome-wide characterization and expression profiling” Agronomy-Basel, December 2021 DOI: https://doi.org/10.3390/agronomy11122511
-
17. Afroz A*, Aslam S, Rashid U, Malik MF, Zeeshan N, Khan MR, But MQS, Khan SA “Intron-containing hairpin RNA interference vector for OBP8 show promising mortality in peach potato aphid” Plant Cell Tissue And Organ Culture, September 2021 DOI: https://doi.org/10.1007/s11240-021-02174-4
-
18. Raza MN, Sughra K, Zeeshan N, Anwar MZ, Shahzad MA, Rashid U, Afroz A, Munir H “Recurrence of hepatitis C virus after treatment with pegylated interferon and direct acting antivirals in Punjab Pakistan” Brazilian Journal Of Biology, July 2021 DOI: https://doi.org/10.1590/1519-6984.252610
-
19. Nadeem MF, Khattak AA, Zeeshan N, Awan UA, Alam S, Ahmad W, Gul S, Afroz A, Sughra K, Rashid U, Khan MA “Molecular Epidemiology of Plasmodium Species in Conflicted Federally Administered Tribal Area of Pakistan” Brazilian Journal Of Biology, May 2021 DOI: https://doi.org/10.1590/1519-6984.247219
-
20. Butt MQ, Zeeshan N, Ashraf NM, Akhtar MA, Ashraf H, Afroz A, Shaheen A, Naz S “Environmental impact and diversity of protease-producing bacteria in areas of leather tannery effluents of Sialkot, Pakistan” Environmental Science And Pollution Research, May 2021 DOI: https://doi.org/10.1007/s11356-021-14477-2
-
21. Afroz A*, Saba Saleem S, Sughra K, Khan SA, Zeeshan N “Identification of candidate biomarkers for HCV leading to hepatocellular carcinoma differential stages from serum samples” Current Pharmaceutical Analysis, February 2020 DOI: https://doi.org/10.2174/1573412914666180912111038
-
22. Manzoor S, Ahmad R, Shahzad M, Sajjad M, Khan N, Rashid U, Afroz A, Khan SA “Salt stress reduces the pea growth and induces the expression of selected antioxidant genes” Pakistan Journal Of Agricultural Sciences, February 2020 DOI: https://doi.org/10.21162/PAKJAS/20.7537
-
23. Hussain T, Rehman N, Inam S, Ajmal W, Afroz A, Muhammad A, Zafar Y, Ali GM, Khan MR “Homotypic clusters of transcription factor binding sites in the first large intron of AGL24 MADS-Box transcription factor are recruited in the enhancement of floral expression” Plant Molecular Biology Reporter, July 2019 DOI: https://doi.org/10.1007/s11105-019-01136-7
-
24. Iqbal T, Rashid U, Shahzad N, Afroz A, Malik MF, Idrees M “Molecular detection of Hepatitis E Virus in layer chickens: A possible public health risk in Pakistan” Pakistan Journal Of Zoology, June 2019 DOI: http://dx.doi.org/10.17582/journal.pjz/2019.51.6.2329.2335
-
25. Iqbal T, Rashid U, Idrees M, Afroz A, Kamili S, Purdy MA “A novel avian isolate of hepatitis E virus from Pakistan” Virology Journal, February 2019 DOI: https://doi.org/10.1186/s12985-019-1247-0
-
26. Iqbal MJ, Ali S, Rashid U, Kamran M, Malik MF, Sughra K, Zeeshan N, Afroz A, Saleem J, Saghir M “Biosynthesis of silver nanoparticles from leave extract of Litchi chinensis and its dynamic biological impact on microbial cells and human cancer cell lines” Cellular And Molecular Biology, October 2018 DOI: https://doi.org/10.14715/cmb/2018.64.13.9
-
27. Sohail W, Majeed F, Afroz A “Differential proteome analysis of diabetes mellitus type 2 and its pathophysiological complications” Diabetes & Metabolic Syndrome-Clinical Research & Reviews, June 2018 DOI: doi.org/10.1016/j.dsx.2018.06.009
-
28. Awais M, Ahmad R, Khan N, Garapati P, Shahzad M, Afroz A, Rashid U, Khan SA “Transformation of tomato variety Riogrande with drought resistant transcription factor gene ATAF1 and its molecular analysis” Pakistan Journal Of Botany, February 2018 DOI: NA
-
29. Rasul F, Afroz A, Rashid U, Mehmood S, Sughra K, Zeeshan N “Screening and characterization of cellulase producing bacteria from soil and waste (molasses) of sugar industry” International Journal of Biosciences, February 2015 DOI: http://dx.doi.org/10.12692/ijb/6.3.230-238
-
30. Nisa FT, Asgher M, Zahid Anwar, Afroz A, Zeeshan N, Rashid U, Irshad M, Mehmood S, Sughra K “Influence of culture conditions on ligninolytic enzymes production from Ganoderma lucidum using agro-industrial residues” Fresenius Environmental Bulletin, December 2014 DOI: NA
-
31. Zahur M, Afroz A, Rashid U, Khaliq S “Dermatomycoses: Challenges and human immune responses” Current Protein & Peptide Science, January 2014 DOI: https://doi.org/10.2174/1389203715666140512121349
-
32. Irshad M, Anwar Z, But HI, Afroz A, Ikram N, Rashid U “The industrial applicability of purified cellulase complex indigenously produced by Trichoderma viride through solid-state bio-processing of agro-industrial and municipal paper wastes” Bioresources, August 2013 DOI: NA
-
33. Khan SA, Hanif Z, Irshad U, Ahmad R, Yasin M, Chaudhary MF, Afroz A, Javed MT, Rashid U, Rashid H “Genetic transformation of sugarcane variety HSF-240 with marker gene GUS” International Journal Of Agriculture And Biology, June 2013 DOI: 83939886
-
34. Afroz A, Zahur M, Zeeshan N, Komatsu S “Plant-bacterium interactions analyzed by proteomics” Frontiers In Plant Science, February 2013 DOI: https://doi.org/10.3389/fpls.2013.00021
-
35. Afroz A, Qureshi A, Rashid U, Zahur M, Nazir F, Iqbal J, Rashid H “Transgenic lines of Oryza sativa (Basmati 2000) expressing Xa21 demonstrate enhanced resistance to bacterial blight” Plant Molecular Biology Reporter, July 2011 DOI: https://doi.org/10.1007/s11105-011-0329-y
-
36. Afroz A, Ali GM, Mir A, Komatsu S “pplication of proteomics to investigate stress-induced proteins for improvement in crop protection” Plant Cell Reports, February 2011 DOI: https://doi.org/10.1007/s00299-010-0982-x
-
37. Afroz A, Chaudhry Z, Rashid U, Khan MR, Ali GM “nhanced regeneration in explants of tomato (Lycopersicon esculentum L.) with the treatment of coconut water” African Journal of Biotechnology, September 2010 DOI: NA
-
38. Afroz A*, Chaudhry Z, Rashid U, Ali GM, Nazir F, Iqbal J, Khan MR “nhanced resistance against bacterial wilt in transgenic tomato (Lycopersicon esculentum cvs) lines expressing the Xa21 gene” Plant Cell Tissue And Organ Culture, August 2010 DOI: https://doi.org/10.1007/s11240-010-9825-2
-
39. Afroz A, Khan MR, Komatsu S “Determination of proteins induced in response to jasmonic acid and salicylic acid in resistant and susceptible cultivars of tomato” Protein And Peptide Letters, July 2010 DOI: https://doi.org/10.1016/j.peptides.2009.06.005
-
40. Afroz A, Hashiguchi A, Khan MR, Komatsu S “Analyses of the proteomes of the leaf, hypocotyl, and root of young soybean seedlings” Protein And Peptide Letters, March 2010 DOI: https://doi.org/10.2174/092986610790780341
-
41. Afroz A, Khan MR, Ahsan N, Komatsu S “Comparative proteomic analysis of bacterial wilt susceptible and resistant tomato cultivars” Peptides, June 2009 DOI: https://doi.org/10.1016/j.peptides
-
42. Amber Afroz, Chaudhry Z, Khan MR, Rashid H, Khan SA “Effect of GA3 on regeneration response of three tomato cultivars (Lycopersicon esculentum M)” Pakistan Journal Of Botany, May 2009 DOI:
-
43. Rapid micro propagation of three elite sugarcane (Saccharum officinarum L.) varieties by shoot tip culture “Khan SA, Rashid H,,Chaudhary MF, Chaudhry Z, Afroz A” African Journal of Biotechnology, July 2008 DOI: NA
-
44. Chaudhry Z, Afroz A, Rashid H “ Effect of variety and plant growth regulators on callus proliferation and regeneration response of three tomato cultivars (Lycopersicon esculentum M.)” Pakistan Journal Of Botany, January 2007 DOI: NA
| Date | Title | Agency/Organization | Amount | Status |
|---|---|---|---|---|
| 2014-09-15 | “Expression analysis and proteomic studies of stress responsive genes” | HEC (PM- IPFP/HRD/HEC/2011/2262) | 0.5 Million Rs | Completed |
| 2024-09-15 | “Proteomic analysis of interaction between a grain aphid and Triticum aestivum cvs to find specific RNAi targets form Gujrat district” | NRPU HEC | 13.3 Million Rs | Completed |